Use the Molecular Diagnostic in the Early Detection of Insect Pests of Stored Grain

Abstract

The current study included early detection of insect pests that infect stored grains using the Multiplex-PCR (Polymerase Chain Reaction) technique. The technique included two experiments conducted using specific primers for each species. These two experiments are as follows: In first experiment three species were identified in wheat grain sample. These species are: an internally infected species Rhyzopertha dominica and two externally infected species Tribolium castaneum and Oryzaephilus mercator . As for the second experiment two species were identified and detected one internally infected Trogoderma granarium and the other externally infected Latheticus oryzae in a red bean sample.